Cytological Study of Anthers for Pollen Sterility in the F7 Generation of a Rice (Oryza sativa L.) Cross

The present research work was proficiently done at the experimental plot of Birsa Agricultural University, Kanke under rainfed conditions Jharkhand, India during kharif 2021. F7 generation seeds cross Pusa-1176 x BPT-5204 sown as panicle to progeny rows in seed bed nursery and thereafter transplanted into field. In this investigation, study has been oriented more towards male sterility it is sensitive than female gametes based on observation recorded previous (F6) regarding spikelet percentage. Henceforth, focussed microscopic pollen grains validate reason behind cause panicles. To understand phenomenon, viability test by staining anthers with 1% Iodine- potassium iodide order know extent fertility or rice segregants. Through study, segregation observed % among families studied which might be due change environmental such temperature, photoperiod humidity etc. expected genetic ratio obtained 9:3:3:1(partially sterile+highly fertile/completely fertile/ highly sterile/completely sterile) revealing that controlled 2 genes since fitted digenic ratio.